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Hot start PCR || principle and usage
4:00
YouTubeAnimated biology With arpan
Hot start PCR || principle and usage
This video describes the principle behind hot start PCR.Hot Start PCR is a technique that inhibits Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Hot Start PCR allows for reaction set up at room temperature without non-specific amplification and primer dimer formation.
31.7K viewsApr 28, 2021
Hot Start PCR Explained
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How Hot-Start PCR Improves PCR Specificity and Reduces Primer Dimers
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