Legislation that goes into effect on Thursday bars UNRWA from operating in the country. That will affect aid delivery to Gaza and beyond.
The law that bans the United Nations agency that aids Palestinian refugees in Gaza went into effect on Wednesday.
The UN has said that its aid agency for Palestinian refugees, UNRWA, will continue working in all Palestinian territories, including east Jerusalem, as Israel cut ties with the organisation.
In the Shuafat refugee camp, a hardscrabble district in east Jerusalem surrounded by a hulking concrete wall, intense security checks make venturing out exasperating.
Israel seeks to deprive Palestine refugees of their right to return to their own land, says Foreign Ministry - Anadolu Ajansı
Follow the latest news headlines from Australia's most trusted source. Read in-depth expert analysis and watch live coverage on ABC News.
Israel has banned a key UN agency from operating in Israel, making the future of its operations in Gaza and the West Bank unclear.
The agency, UNRWA, will be banned from operating on Israeli soil, and contact between it and Israeli officials will also be forbidden.
The directive came in a letter to the U.N., after the Israeli Parliament banned the relief agency that has aided Palestinian refugees for decades.
Laws passed in October went into effect, barring the U.N. agency from the Jewish state and barring state officials from communicating with the group.
The Israeli government ban on UNRWA over national security concerns and links to terrorism began Jan. 30. UNRWA has called the move by Jerusalem as "disastrous."