Nature3d
Efficient lentiviral transduction of liver requires cell cycling in vivo
We transiently transfected 293T and 293TGP cells (a gift from G.P. Nolan) to produce replication-defective HIV-1 lentivirus and MLV, respectively. We seeded 9–12×10 6 cells in 15-cm-diameter ...
Baylor College of Medicine4y
Lentivirus Vectors
These features, in conjunction with several other biosafety enhancements, prevent recombination of replication-competent HIV. All lentivirus vectors produced in the VDL are produced in compliance with ...
The Scientist1y
Bio-Rad Launches Vericheck ddPCR Replication Competent Lentivirus and Replication Competent AAV Kits for Cell and Gene Therapy Production
Bio-Rad Laboratories, Inc. (NYSE: BIO and BIO.B), a global leader in life science research and clinical diagnostics products, today announced the recent launch of the Vericheck ddPCR™ Replication ...
The Scientist8mon
Quality Control for Cell and Gene Therapy
The most common method for evaluating replication competency of lentivirus and AAV vectors is through serial passaging of virus in cell culture lines and subsequent identification of viral particles ...
Nature10y
HIV nuclear import: What's the flap?
In contrast, lentiviruses such as human immunodeficiency ... the nucleus poses a serious challenge to the retroviral replication cycle. This challenge becomes even more daunting if the target ...